paper 8

Selective isolation and distribution of Actinomadura rugatobispora strains in soil

Shin-ichi Suzuki*, Kozo Yamamoto*, Toru Okuda, Maki Nishio*, Noryuki Nakanishi*, and Saburo Komatsubara* *Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd.

Actinomycetol. 14: 27-33, 2000

A simplified enrichment method for selective isolation o Actinomadura rugatobispora strains from soil is described. A. rugatobispora strains poorly produced spores on agar media when 5 to 20 colonies grew on a plate while characteristic spore formation was observed on humic acid-MOPS gellan gum medium. Spores of A. rugatobispora strains were tolerant to dry heating treatment at 120C for 60 min. A. rugatobispora strains were resistant to 50 mg/ml trimethoprim and 10 mg/ml nalidixic acid, both of which inhibited the growth of nonfilamentous soil bacteria. In addition, these strains were more resistant to 100 mg/ml gentamicin and 20 g/ml ampicillin than Streptomyces strains ,which interfere with the growth of A. rugatobispora selectively. Owing these characteristics, we were successful to isolate 260 strains of this species from 148 out of 547 soil samples (27% of the samples tested). They were recovered from the soil samples with pH values ranging from 5.0 to 8.6, and 91% of the strains were isolated from neutral to slightly alkaline soil (pH 6.0 to 9.0). These results indicated that A. rugatobispora strains were distributed worldwide.

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